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KMID : 0376219660030020239
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Chonnam Medical Journal
1966 Volume.3 No. 2 p.239 ~ p.243
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Effects of Nicotinic Acid on the Metabolism of (14)^C-glucose in Rabbit Erythrocytes
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Abstract
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Rabbit erythrocytes were suspended in Krebs-Ringer phosphate buffer (pH 7.4) and incubated at 37°C in the presence of glucose-l-14C, L-glutamine and methylene blue.
The oxygen uptake during incubation. was increased by the addition of 5-amino4-imidazolecarboxamide (ATCA) but decreased by nicotinic acid aloneor even in the presence of AICA by nicotinic acid.
The radioactivities of CO2 and organic anions derived from glucose1-14 were also decreased altogether by nicotinic acid and increased slightly by AICA.
Since in mature erythrocytes CO2 derived from C-1 position of glucose represents the direct oxidative pathway of glucose metabolism, and the radioactivity of organic anions retaining-C-1 position of glucose the Embden-Meyerhof pathway these results were interpreted as indicating that nicotinic acid depresses and AICA enhances both metabolic rathways of glucose although some differences iare noted in their magnitude.
Possible involvement of hexokinase as controlling site of over-all glucose metabolism in erythrocytes and its relation to ATP content of erythrocytes as influenced by nicotinic acid and AICa are discussed.
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